Activating the unit is a simple, user-friendly procedure. If you were to cancel the plan prematurely, a $100.00 termination fee would be applied to your card on file. Because the unit incorporates cellular technology provided by T-Mobile, a service plan, along with a one-year commitment is required under the T-Mobile terms and conditions. Once the account is created, the user will then need to disclose credit card information to keep on file for the monthly fee ($14.99) that will be drawn from the user’s credit card every 30 days. You will also need to provide an alphanumeric name such as Ryan25, Spot04, etc.
Activating an account requires an e-mail address for a username, a password for secure login, a four-digit pin for SMS-based communication with the Zoombak, and the answer to a general security question such as a mother’s maiden name or family pet name. The initial set-up process requires the user to visit the Zoombak website and click on the “activate” tab to create and activate an account. Upon bringing the unit home, you will need to activate the Zoombak GPS tracking unit before it will be ready to record GPS locations. Their message, “We offer products designed to meet everyone’s needs”, leaves an overall aesthetically pleasing first impression on the consumer. What might also catch your eye is the innovative packaging which illustrates Zoombak’s efforts to target specific market segments. Upon purchasing the Zoombak GPS tracking device, you will first notice the conveniently compact size of the unit which comes complete with an AC wall charger, pouch, car charger, and user guide.
MacVectorTip: How to find Restriction Enzymes that only cut outside of a specific region.Simulating DNA electrophoresis in agarose gels using MacVector’s Agarose Gel tool.MacVectorTip: How to copy a specific short amino acid translation of a sequence.If you double-click on the Probable item, you get this editor. The names give some idea of the stringency of the match – Perfect, Probable, Possible and Weak. You can see that the file has four entries – each of these has two segments representing the -35 and -10 region, but each has additional settings that control how close a match has to be before it is reported. If you open the file in MacVector, you can see this. You will find there is an EcoliPromoter.nsub file in the /MacVector/Subsequences/ folder.
For example, the canonical Escherichia coli promoter sequence is a “-35” region TTGACA, then a gap of 16 to 18 residues, then a “-10” region “TATAAT”.
That makes it ideal for searching for bacterial promoters. Each entry in the file MacVector uses as a source of subsequence data can have up to 3 segments, with variable length between the segments, along with a defined number of permitted mismatches and even a system for requiring that specific residues must match. MacVector itself has a built-in variant of the function for maintaining and search primer databases ( Analyze | Primer Database Search…). MacVector’s Subsequence tool is a very flexible search function that can be used for a variety of tasks.